Part:BBa_K5115072

nikA

Introduction

The nikA is a subunit of the nik operon BBa_K2652006.

Usage and Biology

NikA is a periplasmic nickel-binding protein, responsible for sensing and transporting nickel ions into the cell as part of the NikABCDE transporter system. In our design, NikA is constructed into our ribozyme-assisted polycistronic co-expression system BBa_K5115077(ribozyme+RBS+nikA+stem-loop), eventually making up parts BBa_K5115082(ribozyme connected nik operon), which is used for further experiments to facilitate nickel uptake in E. coli.

Characterization

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1560
  
• 1000
  COMPATIBLE WITH RFC[1000]